We studied the transduction mechanisms activated by GTP in PC12 cells and found that addition of GTP (100 uM) increased intracellular calcium concentration ([Ca2+]i) in cells that were between 60 and 70% confluent. Addition of GTP also enhanced activation of NGF-induced extracellular regulated kinases (ERKs) and induced Ca2+ mobilization. This mobilization, due to the activation of voltage-sensitive and ryanodine-sensitive calcium channels, as well as pertussis toxin-sensitive purinoceptors, modulates Ca2-activated K+ channels not involved in activation of ERKs. The results presented here indicate that GTP-triggered [Ca2+]i increase may be a key event in GTP signal transduction, which can modulate activity of ERKs. The physiological importance of the GTP effect lies in its capacity to interact with the NGF-activated pathway to enhance neurite outgrowth from PC12 cells.

Cooperation in signal transduction of extracellular guanosine 5’ triphosphate and Nerve Growth Factor in neuronal differentiation of PC12 cells

GUARNIERI, Simone;FANO' ILLIC', Giorgio;MARIGGIO', Maria Addolorata
2004-01-01

Abstract

We studied the transduction mechanisms activated by GTP in PC12 cells and found that addition of GTP (100 uM) increased intracellular calcium concentration ([Ca2+]i) in cells that were between 60 and 70% confluent. Addition of GTP also enhanced activation of NGF-induced extracellular regulated kinases (ERKs) and induced Ca2+ mobilization. This mobilization, due to the activation of voltage-sensitive and ryanodine-sensitive calcium channels, as well as pertussis toxin-sensitive purinoceptors, modulates Ca2-activated K+ channels not involved in activation of ERKs. The results presented here indicate that GTP-triggered [Ca2+]i increase may be a key event in GTP signal transduction, which can modulate activity of ERKs. The physiological importance of the GTP effect lies in its capacity to interact with the NGF-activated pathway to enhance neurite outgrowth from PC12 cells.
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Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/11564/119844
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