The extreme complexity of biological samples such as whole blood, plasma, serum, urine, saliva demands a simple, fast and robust sample preparation process prior to the instrumental analysis. Conventional sorbent-based sample preparation techniques including solid phase extraction and its different modifications often involve protein precipitation, solvent evaporation and sample reconstitution as the integral part of sample preparation workflow. These extra steps are time consuming and may lead to substantial analyte loss. In order to eliminate these steps from the sample preparation workflow, we have demonstrated the application of fabric phase sorptive extraction (FPSE) to small drugs analyses in biological matrices [1]. Herein, we propose a novel FPSE-HPLC-PDA method for the simultaneous extraction and analysis of three different drugs used in the treatment of inflammatory bowel diseases (IBDs). The wide range of log Kow values ranging from 0.28 for Ciprofloxacin, to 2.1 for Cortisone, and 2.5 for Sulfasalazine pose great challenge for their simultaneous extraction and analysis from biological sample matrices. FPSE, by virtue of its superior extraction efficiency as well as other advantageous attributes, has successfully resolved this issue. During the method development, five different FPSE sorbent chemistries were investigated to select the most efficient sorbent that provides the maximum recovery for all the target drugs. Subsequently, the method was optimized directly in different biological matrices, such as plasma and urine. The validation procedure was carried out following International Conferences of Harmonization (ICH) guidelines [2] in order to demonstrate the viability of the method as a valuable analytical tool for clinical and pharmaceutical applications. Additionally, the new FPSE-HPLC-PDA methodology is simple, fast and green procedure, which can significantly decrease the organic solvents consumption to comply with Green Analytical Chemistry (GAC) approach.

FPSE-HPLC-PDA ANALYSES FOR TRIPLE THERAPY IN THE TREATMENT OF INFLAMMATORY BOWEL DISEASES

LOCATELLI, Marcello;
2017-01-01

Abstract

The extreme complexity of biological samples such as whole blood, plasma, serum, urine, saliva demands a simple, fast and robust sample preparation process prior to the instrumental analysis. Conventional sorbent-based sample preparation techniques including solid phase extraction and its different modifications often involve protein precipitation, solvent evaporation and sample reconstitution as the integral part of sample preparation workflow. These extra steps are time consuming and may lead to substantial analyte loss. In order to eliminate these steps from the sample preparation workflow, we have demonstrated the application of fabric phase sorptive extraction (FPSE) to small drugs analyses in biological matrices [1]. Herein, we propose a novel FPSE-HPLC-PDA method for the simultaneous extraction and analysis of three different drugs used in the treatment of inflammatory bowel diseases (IBDs). The wide range of log Kow values ranging from 0.28 for Ciprofloxacin, to 2.1 for Cortisone, and 2.5 for Sulfasalazine pose great challenge for their simultaneous extraction and analysis from biological sample matrices. FPSE, by virtue of its superior extraction efficiency as well as other advantageous attributes, has successfully resolved this issue. During the method development, five different FPSE sorbent chemistries were investigated to select the most efficient sorbent that provides the maximum recovery for all the target drugs. Subsequently, the method was optimized directly in different biological matrices, such as plasma and urine. The validation procedure was carried out following International Conferences of Harmonization (ICH) guidelines [2] in order to demonstrate the viability of the method as a valuable analytical tool for clinical and pharmaceutical applications. Additionally, the new FPSE-HPLC-PDA methodology is simple, fast and green procedure, which can significantly decrease the organic solvents consumption to comply with Green Analytical Chemistry (GAC) approach.
2017
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Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/11564/668987
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