Phospo-PKCs in Abeta1-42-Specific Human T Cells from Alzheimer’s Disease Patients

Alzheimer's Disease is the most common neurodegenerative dementia of older age. Accurate diagnosis of this condition has important prognostic and therapeutic implications. In the latter, Amyloid-Beta is thought to be produced in excess and subsequently deposited in the brain as plaques, forming the pathological hallmark of Alzheimer's Disease. Interestingly, B- and T-lymphocytes have been implicated in the disease processes being responsible of Amyloid-Bata1-42 peptide removal and activation of inflammation response. Amyloid-Baeta1-42-specific T-cells are present in Alzheimer’s disease but not in other neurodegenerative conditions. By using multi-colour flow-cytometry it is possible to analyse cytokine production and Phosho-Protein-Kinase C expression of in vitro Amyloid-Beta 1-42 stimulated T-cells. It has been demonstrated that a subset of Amyloid-Beta1-42-specific Tcells, characterised by bright expression of Phosphorylated-Protein-Kinase C, distinguishes Alzheimer’s Disease from other neurodegenerative conditions. Therefore, such a new marker might provide further prospective to the studies aimed at diagnosis of Alzheimer’s disease and its discrimination from other forms of dementia.