Keratinocytes are the predominant cells in the epidermis and are involved in the immune response through the secretion of antimicrobial peptides (AMPs). AMPs represent an important evolutionarily conserved innate host-defense mechanism in nearly all organisms. Among the others, human epithelial cells produce mainly β-defensins and chatelicidine. The functions of defensins in immunomodulation, antimicrobial killing and wound repair have been widely investigated in both human and animal models. Epidemiologic and experimental research on the potential effects of extremely low frequency electromagnetic fields (ELF-EMF) on human cells have been performed for a long time. However, there exists a debate on the ability of EMF to influence both inflammatory processes and repair mechanisms including wound healing on different tissue models. We have demonstrated previously that ELF-EMF increases human keratinocyte cell line (HaCaT) proliferation over time exposure. In the present study, to evaluate the effect of ELF-EMF exposure on β-defensins and pro and anti-inflammatory cytokines expression, HaCaT cells have been exposed to 1 mT, 50 Hz for different lengths of time. Gene expression profiles were analyzed by polymerase chain reaction (PCR) in exposed and unexposed control cells. Preliminary results show that IL-1-β and IL-10 cytokines and HBD-2 and HBD-3 expression were time-dependent regulated by ELF-EMF exposure, whereas, ELF-EMF seem to stabilize VDR expression to control the level in all time points. The relationship between IL1-β and HBD-2 and HBD-3 gene expression seem to be more correlated in ELF-EMF exposed HaCaT cells when compared to non-exposed ones. Taken together, the results induce speculation that ELF-EMF, affecting the cytokines and defensins expression, could speed up and improve tissue repair.
KERATINOCYTES AND ANTI MICROBIAL PEPTIDES: INFLUENCE OF EXTREMELY LOW FREQUENCY ELECTROMAGNETIC FIELD (ELF-EMF).
VIANALE, GIOVINA;AURIEMMA, MATTEO;AMERIO, Paolo;REALE, Marcella
2013-01-01
Abstract
Keratinocytes are the predominant cells in the epidermis and are involved in the immune response through the secretion of antimicrobial peptides (AMPs). AMPs represent an important evolutionarily conserved innate host-defense mechanism in nearly all organisms. Among the others, human epithelial cells produce mainly β-defensins and chatelicidine. The functions of defensins in immunomodulation, antimicrobial killing and wound repair have been widely investigated in both human and animal models. Epidemiologic and experimental research on the potential effects of extremely low frequency electromagnetic fields (ELF-EMF) on human cells have been performed for a long time. However, there exists a debate on the ability of EMF to influence both inflammatory processes and repair mechanisms including wound healing on different tissue models. We have demonstrated previously that ELF-EMF increases human keratinocyte cell line (HaCaT) proliferation over time exposure. In the present study, to evaluate the effect of ELF-EMF exposure on β-defensins and pro and anti-inflammatory cytokines expression, HaCaT cells have been exposed to 1 mT, 50 Hz for different lengths of time. Gene expression profiles were analyzed by polymerase chain reaction (PCR) in exposed and unexposed control cells. Preliminary results show that IL-1-β and IL-10 cytokines and HBD-2 and HBD-3 expression were time-dependent regulated by ELF-EMF exposure, whereas, ELF-EMF seem to stabilize VDR expression to control the level in all time points. The relationship between IL1-β and HBD-2 and HBD-3 gene expression seem to be more correlated in ELF-EMF exposed HaCaT cells when compared to non-exposed ones. Taken together, the results induce speculation that ELF-EMF, affecting the cytokines and defensins expression, could speed up and improve tissue repair.I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.
