Recently, Irani and colleagues proposed a C-terminal cleaved isoform cystatin C (12.5kDa) in cerebrospinal fluid as a marker of multiple sclerosis. In this study, we demonstrate that the 12.5kDa product of cystatin C is formed by degradation of the first eight N-terminal residues. Moreover, such a degradation is not specific in the cerebrospinal fluid of multiple sclerosis, but rather is given by an inappropriate sample storage at -20 degrees C. We conclude that the use of the 12.5kDa product of cystatin C in cerebrospinal fluid might lead to a fallacious diagnosis of multiple sclerosis. Preanalytical validation procedure is mandatory for proteomics investigations.

Cleavage of cystatin C is not associated with multiple sclerosis

DEL BOCCIO, PIERO;PIERAGOSTINO, DAMIANA;LUGARESI, Alessandra;DI IOIA, MARIA;PAVONE, Barbara;TRAVAGLINI, DANIELA;SACCHETTA, Paolo;DI ILIO, Carmine;GAMBI, Domenico;URBANI, ANDREA
2007-01-01

Abstract

Recently, Irani and colleagues proposed a C-terminal cleaved isoform cystatin C (12.5kDa) in cerebrospinal fluid as a marker of multiple sclerosis. In this study, we demonstrate that the 12.5kDa product of cystatin C is formed by degradation of the first eight N-terminal residues. Moreover, such a degradation is not specific in the cerebrospinal fluid of multiple sclerosis, but rather is given by an inappropriate sample storage at -20 degrees C. We conclude that the use of the 12.5kDa product of cystatin C in cerebrospinal fluid might lead to a fallacious diagnosis of multiple sclerosis. Preanalytical validation procedure is mandatory for proteomics investigations.
File in questo prodotto:
File Dimensione Formato  
AnnNeu_CysC_20968.pdf

Solo gestori archivio

Tipologia: Documento in Post-print
Dimensione 151.44 kB
Formato Adobe PDF
151.44 kB Adobe PDF   Visualizza/Apri   Richiedi una copia

I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.

Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/11564/108676
Citazioni
  • ???jsp.display-item.citation.pmc??? 6
  • Scopus 42
  • ???jsp.display-item.citation.isi??? 30
social impact