The possibility of entrapping the enzyme carbonic anhydrase into liposomes, in order to obtain small, membrane-confined bioreactors for biotechnological or biomedical applications, was studied. Neutral liposomes (dipalmitoylphosphatidylcholine/cholesterol) or cationic liposomes (dipalmitoylphosphatidylcholine/cholesterol/stearylamine) with different dipalmitoylphosphatidylcholine/stearylamine ratios have been used to trap carbonic anhydrase. Kinetic experiments showed that carbonic anhydrase was being trapped into cationic liposomes, but not into neutral ones. A significant amount of carbonic anhydrase was sitting onto the external surface of liposomes when the ratio dipalmitoylphosphatidylcholine/cholesterol/stearylamine was 6:3:1, but not when it was 5:3:2. Morphological analysis by electron microscopy showed that the presence of carbonic anhydrase induced a significant swelling in the 6:3:1 cationic vesicles, related to the activity of the enzyme.
Interaction of cationic phospholipid vesicles with carbonic anhydrase.
DI MARZIO, Luisa;
1994-01-01
Abstract
The possibility of entrapping the enzyme carbonic anhydrase into liposomes, in order to obtain small, membrane-confined bioreactors for biotechnological or biomedical applications, was studied. Neutral liposomes (dipalmitoylphosphatidylcholine/cholesterol) or cationic liposomes (dipalmitoylphosphatidylcholine/cholesterol/stearylamine) with different dipalmitoylphosphatidylcholine/stearylamine ratios have been used to trap carbonic anhydrase. Kinetic experiments showed that carbonic anhydrase was being trapped into cationic liposomes, but not into neutral ones. A significant amount of carbonic anhydrase was sitting onto the external surface of liposomes when the ratio dipalmitoylphosphatidylcholine/cholesterol/stearylamine was 6:3:1, but not when it was 5:3:2. Morphological analysis by electron microscopy showed that the presence of carbonic anhydrase induced a significant swelling in the 6:3:1 cationic vesicles, related to the activity of the enzyme.I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.
