Cationic liposomes made of dipalmitoylphosphatidylcholine and stearylamine (9: 1) were prepared by reverse-phase evaporation and were able to interact spontaneously with plasmid DNA. The loaded vesicles delivered a P-glucuronidase (GUS)-carrying plasmid to lentil (Lens cufinaris) protoplasts, leading to transient expression of the GUS reporter gene. The transfection efficiency achieved by using stearylamine containing liposomes (lipofection) was comparable to the one obtained by electroporating the protoplasts at 500 pF and different field strengths. Furthermore, the combination of electroporation and lipofection, though reducing cell survival, increased the activity of the reporter enzyme detected in the cell lysates, yielding transient expression levels higher than those recorded after lipofection or electroporation alone
Gene transfer to lentil protoplasts by lipofection and electroporation.
DI MARZIO, Luisa;
1993-01-01
Abstract
Cationic liposomes made of dipalmitoylphosphatidylcholine and stearylamine (9: 1) were prepared by reverse-phase evaporation and were able to interact spontaneously with plasmid DNA. The loaded vesicles delivered a P-glucuronidase (GUS)-carrying plasmid to lentil (Lens cufinaris) protoplasts, leading to transient expression of the GUS reporter gene. The transfection efficiency achieved by using stearylamine containing liposomes (lipofection) was comparable to the one obtained by electroporating the protoplasts at 500 pF and different field strengths. Furthermore, the combination of electroporation and lipofection, though reducing cell survival, increased the activity of the reporter enzyme detected in the cell lysates, yielding transient expression levels higher than those recorded after lipofection or electroporation aloneI documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.
