The purpose of this study was to investigate whether overnight exposure to 1mT–50 Hz extremely low-frequency sinusoidal electromagnetic Weld (EMF) aVects the expression and production of inducible nitric oxide synthase (iNOS) and monocyte chemotactic protein- 1 (MCP-1) in human monocytes. RT-PCR and Western blot analysis demonstrate that EMF exposure aVects the expression of iNOS and MCP-1 in cultured human mononuclear cells at the mRNA level and protein synthesis. Interestingly, the eVects of EMF exposure clearly diVered with respect to the potentiation and inhibition of iNOS and MCP-1 expression. Whereas iNOS was down-regulated both at the mRNA level and at the protein level, MCP-1 was up-regulated. These results provide helpful information regarding the EMF-mediated modulation of the inXammatory response in vivo. However, additional studies are necessary to demonstrate that EMF acts as a nonpharmacological inhibitor of NO and inducer of MCP-1 in some diseases where the balance of MCP-1 and NO may be important.
Modulation of MCP-1 and iNOS by 50 Hz sinusoidal electromagnetic field
REALE, Marcella;DE LUTIIS, Maria Anna;PATRUNO, ANTONIA;SPERANZA, Lorenza;FELACO, Mario;GRILLI, Alfredo;COMANI, Silvia;CONTI, Pio;DI LUZIO, Silvano
2006-01-01
Abstract
The purpose of this study was to investigate whether overnight exposure to 1mT–50 Hz extremely low-frequency sinusoidal electromagnetic Weld (EMF) aVects the expression and production of inducible nitric oxide synthase (iNOS) and monocyte chemotactic protein- 1 (MCP-1) in human monocytes. RT-PCR and Western blot analysis demonstrate that EMF exposure aVects the expression of iNOS and MCP-1 in cultured human mononuclear cells at the mRNA level and protein synthesis. Interestingly, the eVects of EMF exposure clearly diVered with respect to the potentiation and inhibition of iNOS and MCP-1 expression. Whereas iNOS was down-regulated both at the mRNA level and at the protein level, MCP-1 was up-regulated. These results provide helpful information regarding the EMF-mediated modulation of the inXammatory response in vivo. However, additional studies are necessary to demonstrate that EMF acts as a nonpharmacological inhibitor of NO and inducer of MCP-1 in some diseases where the balance of MCP-1 and NO may be important.I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.
