DNA samples from central-eastern Italy were extracted from fresh blood and typed through PCR amplification and automating sequencing. Only 8 haplotypes have been already observed among the Italian populations database available at www.hvrbase.de. The parameters of intrapopulation diversity (haplotype diversity 0.990 +/- 0.008; mean number of pairwise differences 4.979 +/- 2.463) fall into the range of values reported for Italian populations. The values of Tajima (1,944, p = 0.015) and Fu’s tests (-25.552, p = 0.00) are those expected for popula- tions who have had a large and sudden expansion. Applying an exact test for population comparisons implemented in the Arlequin software, we obtained statistically significant p-values for the comparison between our sample and those from Tuscany (0.004) Sardinia (0.039).
Variation of the hypervariable region-1 of mitochondrial DNA in central-eastern Italy
VERGINELLI, Fabio;BATTISTA, Pasquale;MARIANI COSTANTINI, Renato;
2003-01-01
Abstract
DNA samples from central-eastern Italy were extracted from fresh blood and typed through PCR amplification and automating sequencing. Only 8 haplotypes have been already observed among the Italian populations database available at www.hvrbase.de. The parameters of intrapopulation diversity (haplotype diversity 0.990 +/- 0.008; mean number of pairwise differences 4.979 +/- 2.463) fall into the range of values reported for Italian populations. The values of Tajima (1,944, p = 0.015) and Fu’s tests (-25.552, p = 0.00) are those expected for popula- tions who have had a large and sudden expansion. Applying an exact test for population comparisons implemented in the Arlequin software, we obtained statistically significant p-values for the comparison between our sample and those from Tuscany (0.004) Sardinia (0.039).I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.
