The effect of the luminescent heteroaromatic electron acceptor N,N'-dimethyl-2,7-diazapyrenium dichloride (DM-DAP2+) on the stability of 1-palmitoyl-2-oleoylphosphatydilcholine (POPC) liposomes is determined on the basis of the rate of release of different fluorescent probes entrapped within the liposome. The experiments show that DM-DAP2+ exerts a substantial destabilizing action on the liposomal bilayer, particularly at low concentrations. Molecular dynamics simulations suggest that the activity of DM-DAP2+ is related to its tendency to surround itself with water molecules, conceivably favoring the formation of transient pores across the bilayer.
Liposome Destabilization by a 2,7-DiazapyreniumDerivative Through Formation of Transient Pores in theLipid Bilayer
ZAPPACOSTA, ROMINA;FONTANA, Antonella
2010-01-01
Abstract
The effect of the luminescent heteroaromatic electron acceptor N,N'-dimethyl-2,7-diazapyrenium dichloride (DM-DAP2+) on the stability of 1-palmitoyl-2-oleoylphosphatydilcholine (POPC) liposomes is determined on the basis of the rate of release of different fluorescent probes entrapped within the liposome. The experiments show that DM-DAP2+ exerts a substantial destabilizing action on the liposomal bilayer, particularly at low concentrations. Molecular dynamics simulations suggest that the activity of DM-DAP2+ is related to its tendency to surround itself with water molecules, conceivably favoring the formation of transient pores across the bilayer.I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.
