We compared structure and function of EDL and Soleus muscles in adult (4-6 m) mice lacking both Calsequestrin (CASQ) isoforms, the main SR Ca²⁺-binding proteins. Lack of CASQ induced ultrastructural alterations in ~30% of Soleus fibers, but not in EDL. Twitch time parameters were prolonged in both muscles, although tension was not reduced. However, when stimulated for 2 sec at 100 hz, Soleus was able to sustain contraction, while in EDL active tension declined by 70-80%. The results presented in this paper unmask a differential effect of CASQ1&2 ablation in fast versus slow fibers. CASQ is essential in EDL to provide large amount of Ca²⁺ released from the SR during tetanic stimulation. In contrast, Soleus deals much better with lack of CASQ because slow fibers require lower Ca²⁺ amounts and slower cycling to function properly. Nevertheless, Soleus suffers more severe structural damage, possibly because SR Ca²⁺ leak is more pronounced.

Differential effect of calsequestrin ablation on structure and function of fast and slow twitch skeletal fibers.

PAOLINI, CECILIA;PROTASI, Feliciano
2011

Abstract

We compared structure and function of EDL and Soleus muscles in adult (4-6 m) mice lacking both Calsequestrin (CASQ) isoforms, the main SR Ca²⁺-binding proteins. Lack of CASQ induced ultrastructural alterations in ~30% of Soleus fibers, but not in EDL. Twitch time parameters were prolonged in both muscles, although tension was not reduced. However, when stimulated for 2 sec at 100 hz, Soleus was able to sustain contraction, while in EDL active tension declined by 70-80%. The results presented in this paper unmask a differential effect of CASQ1&2 ablation in fast versus slow fibers. CASQ is essential in EDL to provide large amount of Ca²⁺ released from the SR during tetanic stimulation. In contrast, Soleus deals much better with lack of CASQ because slow fibers require lower Ca²⁺ amounts and slower cycling to function properly. Nevertheless, Soleus suffers more severe structural damage, possibly because SR Ca²⁺ leak is more pronounced.
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Utilizza questo identificativo per citare o creare un link a questo documento: http://hdl.handle.net/11564/205532
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