Aim. Aim of this research was to analyse the potential of Amniotic Fluid Stem Cells (AFSCs) to grow, proliferate and differentiate onto implant surfaces subjected to different treatments, Sandblasting, Sandblasting and Large-Grit Acid Etching (SLA), and Full Contact Coverage (FCC). Methods. Machined Ti disks (negative test), Sandblasted Ti disks, SLA Ti disks, and FCC Ti disks were used for this study. The surface micro-topography was observed by SEM and profilometric analysis. AFSCs were withdrawn from sheep in pregnancy. Each sample contains a cell number ranging from 2x103 to 2x106. Pellets of amniotic fluid were directly resuspended in osteogenic medium, without the previous selection of Amniotic Fluid Mesenchymal Stem Cells, on the different Ti disks. At established times, cells on Ti disks were observed by SEM and the presence of calcium deposition in cell culture was observed at light microscope after Alizarin Red staining. Results. At SEM analysis, such as at prophilometer for Ra, Rz and Rsm values, tested surfaces appeared substantially different to each other. At SEM analysis, after 21 days from cell seeding, it was possible to observe how onto machined Ti disks no cell populations were found. Sandblasted Ti disks showed the scarce presence of lengthen and star shaped cells, while numerous cell aggregates were observed onto SLA Ti disks. However, the best results, in term of both cell number and cell differentiation, was obtained by AFCs onto FCC Ti disks, where abundant deposits of mineralized extracellular matrix could be displayed after Alizarin Red Staining. Conclusions. Implant surface micro-topography is known to induce a different response by the host bone tissue. This study showed osteoblast-like cells obtained from amniotic fluid are capable to in vitro colonize Ti surface, but cell activity seems to be strictly connected to the surface micro-topography. Rough surfaces, and in particular FCC, a surface with a controlled roughness, obtained
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