The performance of particle-loaded disks and tradi- tional, packed extraction columns is compared. In this study, solid-phase extraction and liquid chromato- graphic analysis of the anticancer drug mitomycin C are duplicated using two devices that contain chemically similar Cxs bonded silica. The following parameters are used to assess performance: linearity, recovery, preci- sion, and lowest limit of detection. The extraction of mitomycin C from plasma and ultrafiltrate using parti- cle-loaded membranes achieves better precision, im- proved concentration ability, lower detection limits, and greater efficiency. In addiction, elution of drug from the membrane adsorbent is efficiently accomplished using a small volume of mobile phase, eliminating analyte instability problems and the need for additional time- consuming evaporation.
Mitomycin C in biological fluids: Comparison of disk and cartridge solid-phase extraction and quantitation by HPLC in human plasma and ultrafiltrate
CARLUCCI, Giuseppe;MAZZEO, PIETRO;
1999-01-01
Abstract
The performance of particle-loaded disks and tradi- tional, packed extraction columns is compared. In this study, solid-phase extraction and liquid chromato- graphic analysis of the anticancer drug mitomycin C are duplicated using two devices that contain chemically similar Cxs bonded silica. The following parameters are used to assess performance: linearity, recovery, preci- sion, and lowest limit of detection. The extraction of mitomycin C from plasma and ultrafiltrate using parti- cle-loaded membranes achieves better precision, im- proved concentration ability, lower detection limits, and greater efficiency. In addiction, elution of drug from the membrane adsorbent is efficiently accomplished using a small volume of mobile phase, eliminating analyte instability problems and the need for additional time- consuming evaporation.I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.
