A simple and reproducible method for the simultaneous determination of the antimicrobial agent pefloxacin and the anti-inflammatory drug fenbufen with its metabolite felbinac in human plasma is described. It involves a two steps liquid-liquid extraction and separation using an Adsorbosphere SAX column with ultraviolet detection at 280 nm. 2-[4-(2′-furoyl)phenyl]propionic acid is used as internal standard. The coefficients of variation are less than 5%. The lower limits of detection are 0.1, 0.3 and 0.1 μg/mL for pefloxacin, fenbufen and felbinac, respectively. This method was found to be applicable to pharmacokinetic and pharmacodynamic studies of each drug after the concomitant administration of pefloxacin and fenbufen.
Simple and Rapid Analysis of Pefloxacin, Fenbufen and Felbinac in Human Plasma Using High Performance Liquid Chromatography
CARLUCCI, Giuseppe;MAZZEO, PIETRO
1996-01-01
Abstract
A simple and reproducible method for the simultaneous determination of the antimicrobial agent pefloxacin and the anti-inflammatory drug fenbufen with its metabolite felbinac in human plasma is described. It involves a two steps liquid-liquid extraction and separation using an Adsorbosphere SAX column with ultraviolet detection at 280 nm. 2-[4-(2′-furoyl)phenyl]propionic acid is used as internal standard. The coefficients of variation are less than 5%. The lower limits of detection are 0.1, 0.3 and 0.1 μg/mL for pefloxacin, fenbufen and felbinac, respectively. This method was found to be applicable to pharmacokinetic and pharmacodynamic studies of each drug after the concomitant administration of pefloxacin and fenbufen.I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.
