A high - performance liquid chromatographic method for the determination of alfuzosin in human plasma has been developed and validated. A column-switching procedure without extraction was used to isolate the drug from biological matrix prior to the quantitative analysis. The lower limit of detection for the analyte was 1 ng/ml. The method was linear from 2 to 150 ng/ml for human plasma. Within- and between-assay precision and accuracy were all found to be <5.2% at the eight concentrations evaluated. This procedure, simple and rapid, is suitable for pharmacology studies on alfuzosin.

Determination of Alfuzosin in Human Plasma by High-Performance Liquid Chromatography with Column-Switching

CARLUCCI, Giuseppe;MAZZEO, PIETRO
1994-01-01

Abstract

A high - performance liquid chromatographic method for the determination of alfuzosin in human plasma has been developed and validated. A column-switching procedure without extraction was used to isolate the drug from biological matrix prior to the quantitative analysis. The lower limit of detection for the analyte was 1 ng/ml. The method was linear from 2 to 150 ng/ml for human plasma. Within- and between-assay precision and accuracy were all found to be <5.2% at the eight concentrations evaluated. This procedure, simple and rapid, is suitable for pharmacology studies on alfuzosin.
File in questo prodotto:
Non ci sono file associati a questo prodotto.

I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.

Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/11564/453097
 Attenzione

Attenzione! I dati visualizzati non sono stati sottoposti a validazione da parte dell'ateneo

Citazioni
  • ???jsp.display-item.citation.pmc??? ND
  • Scopus ND
  • ???jsp.display-item.citation.isi??? ND
social impact