Ofloxacin is one of the new carboxylic acid derivatives showing a broad antibacterial spectrum active against most Gram negative bacteria, many Gram positive bacteria and some anaerobes. The metabolism of ofloxacin produced 3 metabolites that could interfere with some analytical procedures. In fact, we have compared the HPLC results with a microbiological activity assay showing considerable differences in mean urine levels for the two methods. When measured by bioassay, an increase of urinary recovery between the 4 and 24 hr ofloxacin levels was observed. These differences could suggest a persisting antibacterial activity of some of the metabolic derivatives of ofloxacin.
Comparison of high-performance liquid chromatography and microbiological assay for the determination of ofloxacin in serum and urine in humans
CARLUCCI, Giuseppe;
1990-01-01
Abstract
Ofloxacin is one of the new carboxylic acid derivatives showing a broad antibacterial spectrum active against most Gram negative bacteria, many Gram positive bacteria and some anaerobes. The metabolism of ofloxacin produced 3 metabolites that could interfere with some analytical procedures. In fact, we have compared the HPLC results with a microbiological activity assay showing considerable differences in mean urine levels for the two methods. When measured by bioassay, an increase of urinary recovery between the 4 and 24 hr ofloxacin levels was observed. These differences could suggest a persisting antibacterial activity of some of the metabolic derivatives of ofloxacin.I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.
