Dental Pulp stem cells grown on dental implant titanium surfaces: An in vitro evaluation of differentiation and microRNAs expression

The surface roughness of dental implants influen- ces the proliferation and differentiation rate of adult mesen- chymal stem cells (MSCs). The aim of the present study was to evaluate whether specifically treated titanium implant surfaces influenced human dental pulp stem cells (DPSCs) differentiation in an osteogenic pattern through modulation of microRNAs expression. The degree of differentiation was evaluated after 7, 14, and 21 days, through the expression of microRNAs characterizing the osteogenesis (miR-133 and miR-135), of Runx2 and Smad5 (key factor transcriptions associated with osteoblast differentiation) and Osteocalcin, marker for the bone formation process. DPSCs were cul- tured on sandblasted and acid-etched titanium disks, with (Test) or without the presence of ions (Control). Early differ- entiation of DPSCs cultured on titanium could be detected at all the evaluated time points, respect to cells grown alone. Moreover, the Test surfaces seemed to induce a more marked cells differentiation. The obtained results demon- strated that microRNAs played a pivotal role in the differen- tiation of MSCs and could be used as marker of osteogenic differentiation. Furthermore, the evaluated ionized sand- blastedandacid-etchedsurface enhance the development of osteoblast cells. A faster osseointegration could be achieved in the presence of spe- cifically treated implant surfaces, promising encouraging clinical outcomes.