Objective: The most used techniques to evaluate the inhibitor activity of different compounds on Nitric Oxide Synthases are presented. Method: Griess assay, oxy-hemoglobin capture assay, Radio citrulline assay, and RP-HPLC assay. Result: Griess assay, and oxy-hemoglobin capture assay detect the nitric oxide produced by NOS; while radio citrulline assay, and RP-HPLC assay take into consideration the concentration of L-citrulline as stoichiometric to nitric oxide. Conclusion: Griess assay and oxy-hemoglobin capture assay are suitable for a first screening of NOS activity, while the others could be used for a more precise activity determination.
Methods to Evaluate the Activity of Nitric Oxide Synthase
FANTACUZZI, MARIALUIGIA;AMMAZZALORSO, Alessandra;DE FILIPPIS, Barbara;GIAMPIETRO, Letizia;MACCALLINI, Cristina;AMOROSO, Rosa
2017-01-01
Abstract
Objective: The most used techniques to evaluate the inhibitor activity of different compounds on Nitric Oxide Synthases are presented. Method: Griess assay, oxy-hemoglobin capture assay, Radio citrulline assay, and RP-HPLC assay. Result: Griess assay, and oxy-hemoglobin capture assay detect the nitric oxide produced by NOS; while radio citrulline assay, and RP-HPLC assay take into consideration the concentration of L-citrulline as stoichiometric to nitric oxide. Conclusion: Griess assay and oxy-hemoglobin capture assay are suitable for a first screening of NOS activity, while the others could be used for a more precise activity determination.I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.
