In vitro biological propensities and chemical profiling of Euphorbia milii Des Moul (Euphorbiaceae): A novel source for bioactive agents

Saleem, H.; Zengin, G.; Locatelli, M.; Mollica, A.; Ahmad, I.; Mahomoodally, F. M.; Zainal Abidin, S. A.; Ahemad, N.

doi:10.1016/j.indcrop.2018.12.062

TheplantsofEuphorbiagenushavebeenextensivelystudiedfortheirnutritiveandtherapeuticpurposes.Thepresentresearchistheforemostefforttoinvestigateandcomparethebiologicalactivitiesandchemicalcom-positionofdichloromethane(DCM)andmethanol(MeOH)solventextractsofEuphorbiamilliDesMoulaerialandroot parts. Antioxidant potential was determined using six different (FRAP, CUPRAC, Phosphomolybdenum,DPPH,ABTSandferrouschelation)methods.Theenzymeinhibitioneffectsofthetestedextractswereevaluatedagainst acetylcholinesterase (AChE), butyrylcholinesterase (BChE), α-glucosidase, α-amylase and tyrosinase.Similarly,theamountoftotalphenolicandflavonoidcontentswereassessedviaspectrophotometricmethodsandindividualsecondarymetaboliteswerealsodeterminedusingUHPLC-MSanalysis.MethanolicextractsfrombothaerialandrootpartscontainedthehighestcontentsforphenolicandflavonoidswhichtendstocorrelatewiththeirsignificantDPPH,ABTS(radicalscavenging),FRAP,CUPRAC(reducingpower)andα-glucosidaseinhibitionpotentials.While,boththeDCMextractscontainingthelowestbioactivecontentsweremostactiveinthephosphomolybdenumassay,cholinesterasesandtyrosinaseinhibition.Therootextractsprovedtobeabettersourceofbioactiveantioxidantmolecules.Additionally,UHPLC-MSprofilingofboththemethanolicextractsrevealedthepresenceoftotal16secondarymetabolitesbelongingtofivemajorgroups(phenolic,flavonoid,coumarin,glycosideandalkaloid).Toconclude,ourresultssuggestthatE.miliicanbeconsideredasapromisingleadoriginfornaturalbioactiveenzymeinhibitoryandantioxidantcompoundswhichcouldpavethewayforindustrialapplications

Titolo:	In vitro biological propensities and chemical profiling of Euphorbia milii Des Moul (Euphorbiaceae): A novel source for bioactive agents
Autori:	Saleem H. Zengin G. LOCATELLI, Marcello MOLLICA, ADRIANO Ahmad I. Mahomoodally F. M. Zainal Abidin S. A. Ahemad N. mostra contributor esterni nascondi contributor esterni
Data di pubblicazione:	2019
Rivista:	INDUSTRIAL CROPS AND PRODUCTS
Abstract:	TheplantsofEuphorbiagenushavebeenextensivelystudiedfortheirnutritiveandtherapeuticpurposes.Thepresentresearchistheforemostefforttoinvestigateandcomparethebiologicalactivitiesandchemicalcom-positionofdichloromethane(DCM)andmethanol(MeOH)solventextractsofEuphorbiamilliDesMoulaerialandroot parts. Antioxidant potential was determined using six different (FRAP, CUPRAC, Phosphomolybdenum,DPPH,ABTSandferrouschelation)methods.Theenzymeinhibitioneffectsofthetestedextractswereevaluatedagainst acetylcholinesterase (AChE), butyrylcholinesterase (BChE), α-glucosidase, α-amylase and tyrosinase.Similarly,theamountoftotalphenolicandflavonoidcontentswereassessedviaspectrophotometricmethodsandindividualsecondarymetaboliteswerealsodeterminedusingUHPLC-MSanalysis.MethanolicextractsfrombothaerialandrootpartscontainedthehighestcontentsforphenolicandflavonoidswhichtendstocorrelatewiththeirsignificantDPPH,ABTS(radicalscavenging),FRAP,CUPRAC(reducingpower)andα-glucosidaseinhibitionpotentials.While,boththeDCMextractscontainingthelowestbioactivecontentsweremostactiveinthephosphomolybdenumassay,cholinesterasesandtyrosinaseinhibition.Therootextractsprovedtobeabettersourceofbioactiveantioxidantmolecules.Additionally,UHPLC-MSprofilingofboththemethanolicextractsrevealedthepresenceoftotal16secondarymetabolitesbelongingtofivemajorgroups(phenolic,flavonoid,coumarin,glycosideandalkaloid).Toconclude,ourresultssuggestthatE.miliicanbeconsideredasapromisingleadoriginfornaturalbioactiveenzymeinhibitoryandantioxidantcompoundswhichcouldpavethewayforindustrialapplications
Handle:	http://hdl.handle.net/11564/698974
Appare nelle tipologie:	1.1 Articolo in rivista

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