The etiopathogenesis of neurodegenerative diseases is characterized by the death of neurons. Human periodontal ligament stem cells (hPDLSCs), coming from neuronal crest, can potentially become neuronal cells because of their embryologic origin. In this study, we performed an RNA-seq analysis of hPDLSCs in order to determine whether their transcriptomic profile revealed genes encoded for ion channel receptors. Next, each found gene was enriched by the information of pathways stored in the Reactome database. Our results show that the hPDLSCs express GABBR1 and GABBR2, CHRNA1, GRINA genes, respectively associated with GABAB, NMDA and nACh receptors. In particular, the two subunits of GABAB receptor are expressed in hPDLSCs. Further, the proteic extract for GABABR1, GABABR2 and AChRα1 confirmed their expression in hPDLSCs. Our results show that hPDLSCs express physiologically genes associated with ion channel receptors maintaining multipotent features which are useful for neurogenesis.

Physiological Expression of Ion Channel Receptors in Human Periodontal Ligament Stem Cells

Diomede, Francesca
Secondo
;
Trubiani, Oriana;
2019

Abstract

The etiopathogenesis of neurodegenerative diseases is characterized by the death of neurons. Human periodontal ligament stem cells (hPDLSCs), coming from neuronal crest, can potentially become neuronal cells because of their embryologic origin. In this study, we performed an RNA-seq analysis of hPDLSCs in order to determine whether their transcriptomic profile revealed genes encoded for ion channel receptors. Next, each found gene was enriched by the information of pathways stored in the Reactome database. Our results show that the hPDLSCs express GABBR1 and GABBR2, CHRNA1, GRINA genes, respectively associated with GABAB, NMDA and nACh receptors. In particular, the two subunits of GABAB receptor are expressed in hPDLSCs. Further, the proteic extract for GABABR1, GABABR2 and AChRα1 confirmed their expression in hPDLSCs. Our results show that hPDLSCs express physiologically genes associated with ion channel receptors maintaining multipotent features which are useful for neurogenesis.
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Utilizza questo identificativo per citare o creare un link a questo documento: http://hdl.handle.net/11564/707062
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