The plants of genus Neurada have been utilized for medicinal purposes. This study probed into the biological propensities and endeavored to achieve the detailed phytochemical profile, via ultra-high performance liquid chromatography -UHPLC-MS analysis and high performance liquid chromatography photodiode array -HPLC-PDA of different polarity extracts (methanol, n-hexane, chloroform, and n-butanol) of Neurada procumbens L. The biological features were studied by determining the antioxidant potential via 2,2-Diphenyl-1-picrylhydrazyl -DPPH, 2,2′-azino-bis (3-ethylbenzothiazoline-6-sulphonic acid -ABTS, ferric reducing antioxidant power -FRAP, cupric reducing antioxidant power -CUPRAC, phoshomolybdenum and metal chelation assays and clinically significant major enzymes (cholinesterases, α-amylase, α-glucosidase and tyrosinase inhibition). Moreover, the correlation among the biological activities and total bioactive contents of the extracts were studied via multivariate statistical analysis. The methanol and n-butanol extracts revealed the presence of total phenolic and flavonoid contents in highest concentrations which tend to correlate with their maximum anti-oxidant capacities for radical scavenging and reducing power assays. The n-hexane extract was most active in the phosphomolybdenum assays whereas the chloroform extracts showed the highest metal chelation potential. The UHPLC-MS phytochemical profiling of the methanolic extract (both positive and negative ionization mode) revealed the existence of eighteen phytochemicals representing four different classes (phenolics, flavonoids, sesquiterpenoids, and alkaloids). The n-butanol extract inhibited acetylcholinesterase (4.10 mg galantamine equivalent -GALAE/g) and tyrosinase (127.65 mg kojic acid equivalent -KAE/g) significantly as compared to the control. The n-hexane extract exhibited highest inhibition against butyrylcholinesterase (1.93 mg GALAE/g) and α-glucosidase (59.0 mmol acarbose equivalent -ACAE/g), while chloroform extract exhibited prominent inhibitory action inimical to α-amylase (0.65 mmol ACAE/g). Overall, it was observed that FRAP and tyrosinase activities were the most contributive biological activities for the formation of first component with p values of 0.018 and 0.023, respectively, whereas, for the formation of second component, glucosidase activity was the most contributive assay with p value of 0.002. It can be concluded that the manifestation of dynamic phytochemicals with multi-pharmacological potential marks N. procumbens as a prospective origin of health-promoting molecules warrants further exploration as a unique therapeutic medicinal plant.

Phytochemical composition and in vitro pharmacological investigations of Neurada procumbens L. (Neuradaceae): A multidirectional approach for industrial products

M. Locatelli;
2019

Abstract

The plants of genus Neurada have been utilized for medicinal purposes. This study probed into the biological propensities and endeavored to achieve the detailed phytochemical profile, via ultra-high performance liquid chromatography -UHPLC-MS analysis and high performance liquid chromatography photodiode array -HPLC-PDA of different polarity extracts (methanol, n-hexane, chloroform, and n-butanol) of Neurada procumbens L. The biological features were studied by determining the antioxidant potential via 2,2-Diphenyl-1-picrylhydrazyl -DPPH, 2,2′-azino-bis (3-ethylbenzothiazoline-6-sulphonic acid -ABTS, ferric reducing antioxidant power -FRAP, cupric reducing antioxidant power -CUPRAC, phoshomolybdenum and metal chelation assays and clinically significant major enzymes (cholinesterases, α-amylase, α-glucosidase and tyrosinase inhibition). Moreover, the correlation among the biological activities and total bioactive contents of the extracts were studied via multivariate statistical analysis. The methanol and n-butanol extracts revealed the presence of total phenolic and flavonoid contents in highest concentrations which tend to correlate with their maximum anti-oxidant capacities for radical scavenging and reducing power assays. The n-hexane extract was most active in the phosphomolybdenum assays whereas the chloroform extracts showed the highest metal chelation potential. The UHPLC-MS phytochemical profiling of the methanolic extract (both positive and negative ionization mode) revealed the existence of eighteen phytochemicals representing four different classes (phenolics, flavonoids, sesquiterpenoids, and alkaloids). The n-butanol extract inhibited acetylcholinesterase (4.10 mg galantamine equivalent -GALAE/g) and tyrosinase (127.65 mg kojic acid equivalent -KAE/g) significantly as compared to the control. The n-hexane extract exhibited highest inhibition against butyrylcholinesterase (1.93 mg GALAE/g) and α-glucosidase (59.0 mmol acarbose equivalent -ACAE/g), while chloroform extract exhibited prominent inhibitory action inimical to α-amylase (0.65 mmol ACAE/g). Overall, it was observed that FRAP and tyrosinase activities were the most contributive biological activities for the formation of first component with p values of 0.018 and 0.023, respectively, whereas, for the formation of second component, glucosidase activity was the most contributive assay with p value of 0.002. It can be concluded that the manifestation of dynamic phytochemicals with multi-pharmacological potential marks N. procumbens as a prospective origin of health-promoting molecules warrants further exploration as a unique therapeutic medicinal plant.
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Utilizza questo identificativo per citare o creare un link a questo documento: http://hdl.handle.net/11564/711817
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