A two site solid phase radioimmunoassay for detection of common food antigens is described. Bovine serum albumin, β-lactoglobulin and ovalbumin can be detected in normal human serum at levels ranging from 0.1 to >1000 ng/ml; sensitivity is not impaired by the presence of low levels of antibodies. Thirty min to 3 h after oral intake of milk, β-lactoglobulin could be detected in the sera of 3 normal individuals, at a concentration of 0.1-3 ng/ml. This assay should prove useful in assessing the importance of macromolecular absorption in food allergy and in other gastrointestinal diseases. © 1980.
Solid phase radioimmunoassay for detection of circulating food protein antigens in human serum
Paganelli R.;
1980-01-01
Abstract
A two site solid phase radioimmunoassay for detection of common food antigens is described. Bovine serum albumin, β-lactoglobulin and ovalbumin can be detected in normal human serum at levels ranging from 0.1 to >1000 ng/ml; sensitivity is not impaired by the presence of low levels of antibodies. Thirty min to 3 h after oral intake of milk, β-lactoglobulin could be detected in the sera of 3 normal individuals, at a concentration of 0.1-3 ng/ml. This assay should prove useful in assessing the importance of macromolecular absorption in food allergy and in other gastrointestinal diseases. © 1980.File in questo prodotto:
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