After oral mucosal injury, the healing response following specific steps that lead to wound closure and to tissue repair. Multiple cell populations are involved in this process; in particular, fibroblasts play a key role in the production of extracellular matrix (ECM). During wound healing the remodeling of ECM is a key stage to restore the tissue functionality through multifunctional fibroblast populations that are placed in the connective tissues of gingiva and periodontal ligament. Notably, a fibroblast sub-type (myofibroblast) is centrally involved in collagen synthesis and fibrillar remodeling. The present work evidenced the role of Transforming Growth Factor-beta1 (TGF-beta 1) to mediate human gingival fibroblasts (hGFs) differentiation into myofibroblasts derived from gingival fibroblasts (myo-hGFs). The morphological and functional features were analyzed through Confocal Laser Scanning Microscopy (CLSM), flow cytometry, and western blotting analyses. The specific markers, such as alpha-Smooth Muscle Actin (alpha-SMA), Vimentin, E-cadherin, beta-catenin, and Smad 2/3, were modulated in myo-hGFs after the induction with TGF-beta 1, at different time points (24, 48, and 72 h). After 72 h of treatment TGF-beta 1 operates as an inducer of hGFs into myo-hGFs differentiation. We propose that TGF-beta 1 may promote in vitro the fibroblasts-to-myofibroblasts transition via the morphological and molecular modifications, as the induction of alpha-SMA, Vimentin, E-cadherin, beta-catenin, and Smad 2/3.

Transforming Growth Factor-Beta1 and Human Gingival Fibroblast-to-Myofibroblast Differentiation: Molecular and Morphological Modifications

Marconi, Guya D
Primo
;
Fonticoli, Luigia;Lanuti, Paola;Della Rocca, Ylenia;Pierdomenico, Sante D;Trubiani, Oriana
;
Pizzicannella, Jacopo
Penultimo
;
Diomede, Francesca
Ultimo
2021-01-01

Abstract

After oral mucosal injury, the healing response following specific steps that lead to wound closure and to tissue repair. Multiple cell populations are involved in this process; in particular, fibroblasts play a key role in the production of extracellular matrix (ECM). During wound healing the remodeling of ECM is a key stage to restore the tissue functionality through multifunctional fibroblast populations that are placed in the connective tissues of gingiva and periodontal ligament. Notably, a fibroblast sub-type (myofibroblast) is centrally involved in collagen synthesis and fibrillar remodeling. The present work evidenced the role of Transforming Growth Factor-beta1 (TGF-beta 1) to mediate human gingival fibroblasts (hGFs) differentiation into myofibroblasts derived from gingival fibroblasts (myo-hGFs). The morphological and functional features were analyzed through Confocal Laser Scanning Microscopy (CLSM), flow cytometry, and western blotting analyses. The specific markers, such as alpha-Smooth Muscle Actin (alpha-SMA), Vimentin, E-cadherin, beta-catenin, and Smad 2/3, were modulated in myo-hGFs after the induction with TGF-beta 1, at different time points (24, 48, and 72 h). After 72 h of treatment TGF-beta 1 operates as an inducer of hGFs into myo-hGFs differentiation. We propose that TGF-beta 1 may promote in vitro the fibroblasts-to-myofibroblasts transition via the morphological and molecular modifications, as the induction of alpha-SMA, Vimentin, E-cadherin, beta-catenin, and Smad 2/3.
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Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/11564/753866
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