Magnetic solid phase extraction (MSPE) coupled to high performance liquid chromatography (HPLC)–diode array detection(DAD) method has been developed for the sensitive analysis of Anastrozole (ANA) and Letrozole (LET) in human urine samples. Characterization studies of the newly synthesized material (Fe3O4@TEPA) were carried out by Fourier–transform infrared spectroscopy (FTIR), X–ray diffraction (XRD), Raman spectroscopy, and scanning electron microscope (SEM) techniques. The method has provided linearity for both the analytes (r2 > 0.9900) in the range of 4.00–160.00 ng mL−1. The limit of detections (LODs) for ANA and LET were found to be 1.14 and 1.26 ng mL-1, respectively. The limit of quantification (LOQs) for ANA and LET were obtained 3.78 and 3.86 ng mL-1, respectively. The new method was simple, convenient, fast, and suitable for the direct analysis of ANA and LET in urine samples. Finally, the developed method was applied to the spiked human urine samples to test accuracy and repeatability. Recovery values for each of the molecules were calculated in the range of 93.4–108.0 % (n = 3) at two concentration levels, while % RSDs were lower than 6.2 %.
Sensitive determination of Anastrozole and Letrozole in urine samples by novel magnetic nanoparticles containing tetraethylenepentamine (TEPA) prior to analysis by high performance liquid chromatography-diode array detection
Marcello Locatelli;Angela Tartaglia;
2022-01-01
Abstract
Magnetic solid phase extraction (MSPE) coupled to high performance liquid chromatography (HPLC)–diode array detection(DAD) method has been developed for the sensitive analysis of Anastrozole (ANA) and Letrozole (LET) in human urine samples. Characterization studies of the newly synthesized material (Fe3O4@TEPA) were carried out by Fourier–transform infrared spectroscopy (FTIR), X–ray diffraction (XRD), Raman spectroscopy, and scanning electron microscope (SEM) techniques. The method has provided linearity for both the analytes (r2 > 0.9900) in the range of 4.00–160.00 ng mL−1. The limit of detections (LODs) for ANA and LET were found to be 1.14 and 1.26 ng mL-1, respectively. The limit of quantification (LOQs) for ANA and LET were obtained 3.78 and 3.86 ng mL-1, respectively. The new method was simple, convenient, fast, and suitable for the direct analysis of ANA and LET in urine samples. Finally, the developed method was applied to the spiked human urine samples to test accuracy and repeatability. Recovery values for each of the molecules were calculated in the range of 93.4–108.0 % (n = 3) at two concentration levels, while % RSDs were lower than 6.2 %.I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.
