The effect of casein phosphopeptide-amorphous calcium phosphate on chemical-induced enamel erosion: an in vitro study with ESEM analysis

AIM: Chemical exogen acids effect can be considered in terms of the loss of hard tissue of teeth or potential loss. When the remineralization does not antagonize the demineralization, the dissolution of hard tissues arises. Significant among physical processes is degeneration in enamel structure due to the chemical processes of acidification and alkalinization. There are also several underlying determinants of enamel erosion. The enamel erosion induced by chemical exogen acids causes the dissolution of the hard tissues of teeth. The aim of this study was to analyze the erosive effect of three different soft drinks on the enamel surface and the potential remineralizing effect after applying casein phosphopeptide-amorphic calcium phosphate. MATERIALS AND METHODS: Fifteen human third molar teeth specimens were analyzed to assess the effects of the topical cream containing 10% w/w casein phosphopeptide-amorphic calcium phosphate (CPP-ACP) nanocomplexes on enamel teeth lesions. Morphological characterization of teeth was performed using Environmental Scanning Electron Microscope (ESEM). In addition, the surface of specimens was examined before and after immersion into three recipients containing three different soft drinks and after the remineralization process for comparison. RESULTS: Environmental Scanning Electron Microscopy analysis showed enamel morphology alterations after acidic soft drink exposure and superficially repair of teeth enamel after remineralizing treatment. CONCLUSION: This in vitro study demonstrated that the casein phosphopeptide-amorphic calcium phosphate treatment was effective in remineralizing demineralized subsurface lesion enamel in vitro after having caused alterations by exogen acids.