Chronic kidney disease (CKD) is a complex condition that affects multiple organs and often coexists with conditions such as lupus, diabetes mellitus, cardiovascular disease, and chronic inflammation. The conventional biomarker, serum creatinine, is hindered by its variability and limited sensitivity in accurately assessing renal function. Metabolites derived from the tricarboxylic acid (TCA) cycle and respiratory enzymes, notably succinate, play a crucial role in the progression of CKD. Succinate is an activator of the renin-angiotensin system (RAS), a pivotal mechanism in renal diseases. Taking into account its role in RAS and the presence of succinate in urine, it has emerged as a promising noninvasive biomarker for detecting CKD. This study focuses on developing a boron-doped carbon nitride nanosheets (B-CNNS) impedimetric sensor to detect succinate ions in artificial urine, with the aim of enabling early detection of CKD. The BCN-based sensor demonstrates an enhanced sensitivity compared to existing CKD sensors, achieving a Limit of Detection (LOD) of 0.025 pM and a Limit of Quantification (LOQ) of 0.075 pM. These results highlight the potential of BCN-based sensors for efficient and early succinate detection, presenting promising avenues for advancing CKD management. This research underscores the significance of succinate as a biomarker for the diagnosis of CKD and advocates for increased recognition of its diagnostic utility in clinical trials.
Ultrasensitive impedimetric detection of Succinate- A biomarker for Chronic kidney disease on Picomolar level using boron doped carbon nitride nanosheet
Bhabal, Manasi;
2025-01-01
Abstract
Chronic kidney disease (CKD) is a complex condition that affects multiple organs and often coexists with conditions such as lupus, diabetes mellitus, cardiovascular disease, and chronic inflammation. The conventional biomarker, serum creatinine, is hindered by its variability and limited sensitivity in accurately assessing renal function. Metabolites derived from the tricarboxylic acid (TCA) cycle and respiratory enzymes, notably succinate, play a crucial role in the progression of CKD. Succinate is an activator of the renin-angiotensin system (RAS), a pivotal mechanism in renal diseases. Taking into account its role in RAS and the presence of succinate in urine, it has emerged as a promising noninvasive biomarker for detecting CKD. This study focuses on developing a boron-doped carbon nitride nanosheets (B-CNNS) impedimetric sensor to detect succinate ions in artificial urine, with the aim of enabling early detection of CKD. The BCN-based sensor demonstrates an enhanced sensitivity compared to existing CKD sensors, achieving a Limit of Detection (LOD) of 0.025 pM and a Limit of Quantification (LOQ) of 0.075 pM. These results highlight the potential of BCN-based sensors for efficient and early succinate detection, presenting promising avenues for advancing CKD management. This research underscores the significance of succinate as a biomarker for the diagnosis of CKD and advocates for increased recognition of its diagnostic utility in clinical trials.I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.
