Aim. Calcium sulphate (CaS) is an alloplastic material that, after resorption, leaves behind a calcium phosphate lattice which promotes bone regeneration. The aim of this study was a histological and an ultrastructural evaluation of the bone formed in human sinus augmentation procedures with CaS. Methods. Twenty specimens retrieved from the sinus after a healing period of 6 months were investigated. SEM and energy dispersive X-ray spectroscopy (EDS) was used to evaluate the relationship between CaS and newly-formed bone. Birefringence was used to evaluate the bone structure around the CaS particles by polarized light microscopy (CPLM). Unstained sections were also investigated using the fluorescence (FM) in reflected UV light to evaluate the interface between CaS and newly-formed bone. Results. EDS analysis of 6 specimens showed that very little sulphur remained and residual particles appear to have transformed into calcium phosphate. Under CPLM a few biomaterial remnants were present in some areas and covered by mature bone. The collagen fibers inside both mineralized bone matrix and osteoid adjacent to the particles appeared mostly randomly oriented. Under FM it was possible to observe the relationship between residual particles and bone. Conclusions. The present results confirm the high biocompatibility, rapid resorption rate, and the mechanism of transformation of CaS to calcium phosphate
Bone regeneration in sinus augmentation procedures with calcium sulfate evaluated underscanning, fluorescence and circularly polarized light microscopy. a human study
SINJARI, BRUNA;TRAINI, TONINO
2011-01-01
Abstract
Aim. Calcium sulphate (CaS) is an alloplastic material that, after resorption, leaves behind a calcium phosphate lattice which promotes bone regeneration. The aim of this study was a histological and an ultrastructural evaluation of the bone formed in human sinus augmentation procedures with CaS. Methods. Twenty specimens retrieved from the sinus after a healing period of 6 months were investigated. SEM and energy dispersive X-ray spectroscopy (EDS) was used to evaluate the relationship between CaS and newly-formed bone. Birefringence was used to evaluate the bone structure around the CaS particles by polarized light microscopy (CPLM). Unstained sections were also investigated using the fluorescence (FM) in reflected UV light to evaluate the interface between CaS and newly-formed bone. Results. EDS analysis of 6 specimens showed that very little sulphur remained and residual particles appear to have transformed into calcium phosphate. Under CPLM a few biomaterial remnants were present in some areas and covered by mature bone. The collagen fibers inside both mineralized bone matrix and osteoid adjacent to the particles appeared mostly randomly oriented. Under FM it was possible to observe the relationship between residual particles and bone. Conclusions. The present results confirm the high biocompatibility, rapid resorption rate, and the mechanism of transformation of CaS to calcium phosphateI documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.
