Goji (Lycium barbarum L.) berries have been widely used as a medicinal plant as well as in the preparation of different phytopharmaceuticals [1]. In this study the antioxidant capacity, enzyme inhibitory and cytotoxic effects of two newly developed Goji cultivars (Erma and Biglifeberry) were tested as well as their phytochemical composition by HR-ESI-QToF-MS. Antioxidant capacity was assessed with different assays including free radical scavenging (ABTS and DPPH), reducing power (FRAP and CUPRAC), phosphomolybdenum and metal chelating assays. Enzyme inhibitory effects were tested against cholinesterase, tyrosinase, amylase and glucosidase. iCELLigence real time cell analysis system was used to determine the cytotoxic activity of extracts from these cultivars on HEK-293 cells. HR-ESI-QToF-MS revealed a slightly different chemical composition of the cultivars. However, Biglifeberry extract exhibited stronger antioxidant abilities with higher levels of total phenolics () compared to the cultivar Erma (). However, Erma extract had higher inhibitory effects on butyrylcholinesterase, glucosidase and tyrosinase. Moreover, these extracts showed no cytotoxic effects on HEK-293 cells in a time and dose dependent manner. LC-MS results showed the presence of several compounds including important vitamin C and dietary flavonoids, such as rutin and phenolic acids such as coumaroyl and caffeoyl quinic acid derivatives and characteristic lycibarbarspermidines in both cultivars. These findings suggest that the two Goji cultivars could be considered as potential sources of bioactive compounds for the design of novel functional and health-promoting phytopharmaceuticals. Acknowledgements: References: [1] Mocan A, Zengin G, Simirgiotis M, Schafberg M, Mollica A, Vodnar DC, Crişan G, Rohn S. (2017). Functional constituents of wild and cultivated Goji (L. barbarum L.) leaves: phytochemical characterization, biological profile, and computational studies. Journal of Enzyme Inhibition and Medicinal Chemistry, 32: 153-168.
HR-ESI-QToF-MS characterization of bioactive components from two Goji (Lycium barbarum L.) berries cultivar and their antioxidant, enzyme inhibitory and toxicological evaluation
LOCATELLI, Marcello;
2017-01-01
Abstract
Goji (Lycium barbarum L.) berries have been widely used as a medicinal plant as well as in the preparation of different phytopharmaceuticals [1]. In this study the antioxidant capacity, enzyme inhibitory and cytotoxic effects of two newly developed Goji cultivars (Erma and Biglifeberry) were tested as well as their phytochemical composition by HR-ESI-QToF-MS. Antioxidant capacity was assessed with different assays including free radical scavenging (ABTS and DPPH), reducing power (FRAP and CUPRAC), phosphomolybdenum and metal chelating assays. Enzyme inhibitory effects were tested against cholinesterase, tyrosinase, amylase and glucosidase. iCELLigence real time cell analysis system was used to determine the cytotoxic activity of extracts from these cultivars on HEK-293 cells. HR-ESI-QToF-MS revealed a slightly different chemical composition of the cultivars. However, Biglifeberry extract exhibited stronger antioxidant abilities with higher levels of total phenolics () compared to the cultivar Erma (). However, Erma extract had higher inhibitory effects on butyrylcholinesterase, glucosidase and tyrosinase. Moreover, these extracts showed no cytotoxic effects on HEK-293 cells in a time and dose dependent manner. LC-MS results showed the presence of several compounds including important vitamin C and dietary flavonoids, such as rutin and phenolic acids such as coumaroyl and caffeoyl quinic acid derivatives and characteristic lycibarbarspermidines in both cultivars. These findings suggest that the two Goji cultivars could be considered as potential sources of bioactive compounds for the design of novel functional and health-promoting phytopharmaceuticals. Acknowledgements: References: [1] Mocan A, Zengin G, Simirgiotis M, Schafberg M, Mollica A, Vodnar DC, Crişan G, Rohn S. (2017). Functional constituents of wild and cultivated Goji (L. barbarum L.) leaves: phytochemical characterization, biological profile, and computational studies. Journal of Enzyme Inhibition and Medicinal Chemistry, 32: 153-168.I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.
