Targeting bladder cancer aggressiveness: an in vitro study of the effects of a Multi-Strain Probiotic Formulation lysate

Bladder cancer (BC) is one of the most prevalent malignancies of the urinary tract, characterized by high recurrence rates and significant morbidity. Recently, oxidative stress and chronic inflammation have emerged as central contributors to bladder carcinogenesis. Consequently, modulating oxidative stress has emerged as a promising therapeutic strategy, especially through the use of redox-active compounds that restore cellular homeostasis or sensitize cancer cells to treatment. In our study, we investigated the effects of OxxySlab (a multistrain probiotic formulation) on bladder cancer cell lines, focusing on its impact on cell viability and proliferation, clonogenic potential, cell migration, ROS production and antioxidant defense systems, EMT marker expression, cellular senescence. Our findings demonstrating therapeutic potential of multispecies probiotics can selectively induce oxidative stress in cancer cells, promoting senescence and inhibiting malignancy progression.