Sage species are used in Romanian traditional medicine for coughs, rheumatism and inflammatory diseases as well as antidiabetic remedies. In this study Salvia glutinosa and the endemic Salvia transsylvanica were compared with the well known Salvia officinalis in terms of chemical composition and biological activities. Previously validated HPLC method was applied for determination of 22 phenolic compounds in dry extracts of S. glutinosa, S. transsylvanica, and S. officinalis,. In the samples the total amount of phenolics was 6675.9, 4937.7 and 8425.5 μg g-1, respectively. The chromatographic fingerprint revealed that the main compounds of Salvia species are rutin (1357.9-4070.2 μg g-1) and catechin (1112.6-1911.1 μg g-1). The biological profile of the three sage extracts was evaluated towards several antioxidant assays as well as by testing their inhibitory capacities against hey enzymes involved in neurodegenerative diseases as well as diabetes mellitus. Concerning the antioxidant assays, generally S. officinalis exhibited high antioxidant capacity in all assays, the highest values being obtained for the CUPRAC assay: 400.01 mgTE/g extract for S. officinalis, 175.91 mgTE/g extract for S. glutinosa, and 118.11 mgTE/g extract for S. transsylvanica. Concerning the enzyme inhibitory assays, S. officinalis extract presented the highest inhibitory potential on butyrylcholinesterase (2.40 mgGALAE/g extract) followed by S. transsylvanica (1.43 mgGALAE/g extract). Additionally, both S. officinalis and S. transsylvanica extracts exhibited an important inhibitory potential against alpha-glucosidase (27.01 mmolACAE/g extract, and 25.62 mmolACAE/g extract, respectively). Acknowledgments Authors acknowledge for financial support MIUR ex 60%, University “G. d’Annunzio” of Chieti – Pescara, Chieti, Italy, Scientific Grant Agency of the MESRS of the Slovak Republic (VEGA 1/0010/15).
Determination of phenolic compounds in different species of Salvia (Salvia trnssylvanica, Salvia officinalis, Salvia glutinosa) using HPLC-PDA analysis and their biological activities
CARRADORI, Simone;LOCATELLI, Marcello;
2017-01-01
Abstract
Sage species are used in Romanian traditional medicine for coughs, rheumatism and inflammatory diseases as well as antidiabetic remedies. In this study Salvia glutinosa and the endemic Salvia transsylvanica were compared with the well known Salvia officinalis in terms of chemical composition and biological activities. Previously validated HPLC method was applied for determination of 22 phenolic compounds in dry extracts of S. glutinosa, S. transsylvanica, and S. officinalis,. In the samples the total amount of phenolics was 6675.9, 4937.7 and 8425.5 μg g-1, respectively. The chromatographic fingerprint revealed that the main compounds of Salvia species are rutin (1357.9-4070.2 μg g-1) and catechin (1112.6-1911.1 μg g-1). The biological profile of the three sage extracts was evaluated towards several antioxidant assays as well as by testing their inhibitory capacities against hey enzymes involved in neurodegenerative diseases as well as diabetes mellitus. Concerning the antioxidant assays, generally S. officinalis exhibited high antioxidant capacity in all assays, the highest values being obtained for the CUPRAC assay: 400.01 mgTE/g extract for S. officinalis, 175.91 mgTE/g extract for S. glutinosa, and 118.11 mgTE/g extract for S. transsylvanica. Concerning the enzyme inhibitory assays, S. officinalis extract presented the highest inhibitory potential on butyrylcholinesterase (2.40 mgGALAE/g extract) followed by S. transsylvanica (1.43 mgGALAE/g extract). Additionally, both S. officinalis and S. transsylvanica extracts exhibited an important inhibitory potential against alpha-glucosidase (27.01 mmolACAE/g extract, and 25.62 mmolACAE/g extract, respectively). Acknowledgments Authors acknowledge for financial support MIUR ex 60%, University “G. d’Annunzio” of Chieti – Pescara, Chieti, Italy, Scientific Grant Agency of the MESRS of the Slovak Republic (VEGA 1/0010/15).I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.
