Biofilm represents a successful strategy for Helicobacter pylori survival. Current studies report that the EPS matrix of H. pylori biofilm is mainly constituted by proteomannans, LPS-related structures, extracellular DNA (eDNA), proteins and Outer Membrane Vesicles (OMVs). The OMVs are spherical bilayered structures (20-250 nm in diameter) which are released by the microorganisms during their growth and have a key role in H. pylori biofilm formation. A recent study analyzed the exoproteome of H. pylori at different growth phases in the planktonic phenotype. Among the 74 proteins selectively released by OMVs, the Carbonic Anhydrase (CA) was identified, however, no indication of the family was provided. Intriguing, the pathogen uses its CAs belonging to the alpha- and beta-families for the acid acclimation within the human stomach and thus, for the bacterial survival in the host. The aim of the present study was the detection and characterization of CA in the OMVs generated in the planktonic and biofilm phenotypes over time by H. pylori.

Detection and Characterization of Carbonic Anhydrases in the Outer Membrane Vesicles (OMVs) Released by Helicobacter pylori in the Planktonic and Biofilm Phenotypes

Rossella Grande
;
Maurizio Ronci;Valentina Puca;Simone Carradori;Raffaella Muraro;Claudiu Supuran;
2018

Abstract

Biofilm represents a successful strategy for Helicobacter pylori survival. Current studies report that the EPS matrix of H. pylori biofilm is mainly constituted by proteomannans, LPS-related structures, extracellular DNA (eDNA), proteins and Outer Membrane Vesicles (OMVs). The OMVs are spherical bilayered structures (20-250 nm in diameter) which are released by the microorganisms during their growth and have a key role in H. pylori biofilm formation. A recent study analyzed the exoproteome of H. pylori at different growth phases in the planktonic phenotype. Among the 74 proteins selectively released by OMVs, the Carbonic Anhydrase (CA) was identified, however, no indication of the family was provided. Intriguing, the pathogen uses its CAs belonging to the alpha- and beta-families for the acid acclimation within the human stomach and thus, for the bacterial survival in the host. The aim of the present study was the detection and characterization of CA in the OMVs generated in the planktonic and biofilm phenotypes over time by H. pylori.
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Utilizza questo identificativo per citare o creare un link a questo documento: http://hdl.handle.net/11564/697669
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