A Scalable Tangential Flow Filtration Platform for High-Yield Isolation of Plant-Derived Vesicles from Premium Botanical Sources.

Plant-derived vesicles (PDVs) obtained from premium botanical sources such as grapes, bergamot, argan, artichokes, and olive leaves represent an innovative and disruptive frontier for cosmetics, nutraceuticals, and next-generation therapies. We developed a highly efficient, versatile, and industry-ready tangential flow filtration (TFF) platform capable of isolating and scaling up the production of many different plant matrices while minimizing shear stress; the system ensures unprecedented batch-to-batch consistency and a massive increase by several orders of magnitude in vesicle recovery rates 35.8% (average of all samples), without compromising structural integrity. We have successfully achieved PDV extraction and purification from various plant components, including juice, pulp, leaves, peels, and seeds. Comprehensive physicochemical, molecular, and high-throughput proteomic characterization of these botanical isolates confirms that our optimized TFF process based on specific molecular weight cut-off values consistently yields structurally intact and highly stable nanostructures within the size ranges reported for PDVs 192±nm (average of all samples); these structures preserve cell-specific surface markers and a rich bioactive protein cargo. This optimized workflow marks the transition of PDV technology from basic research to a scalable, turnkey solution, offering a highly adaptable extraction method that provides companies and Researchers with immediate access to high-purity, large-scale bioactive nanoplatforms ready for commercial formulation.